アルケニルコハク酸無水物(ASA)によるリパーゼの化学修飾

概要

論文の詳細を見る
The amino groups of lipase-P (Pseudomonas sp. lipase from Amano Pharmaceutical Ltd.) were modified with alkenyl succinic anhydride (ASA) that is used as a reactive sizing agent for paper making in industry. It was possible to N-acylate the amino groups smoothly up to 50% modification with little inactivation of the enzyme, using emulsified tetradecenyl succinic anhydride at 37°C, pH 7.5. Ester-synthesis activity of the enzymes was estimated by using oleic acid and oleyl alcohol as substrates in cyclohexane. The enzymes were suspended in the substrate solution in powder form or in immobilized enzyme form (immobilized on celite and macroporous ion exchange resin). Both enzymes resulted in higher ester-synthesis activity. The initial ester-synthesis rate was 8.4 times that of either enzyme in its native form.
社団法人日本油化学会の論文