低温環境のマウス抗体産生能に及ぼす影響
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概要
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The influence of low ambient temperatures on the immune response was studied in mice. Male dd-strain mice weighing about 15g (4 weeks of age), were raised in a climatic chamber, 25±1°C and 60% relative humidity, with light from 0700 to 1900 hours (LD 12:12). After 3 weeks, the mice were randomly divided into control and the experimental groups, and were immunized with a single injection of sheep red blood cells (6.6×107/g body weight). Just after immunization, the experimental groups were transferred into one of the following climatic chambers maintained at 18, 15, 10, 8, 4, 2, or 1°C. The control group was not moved. All mice were sacrificed to collect sera and spleen samples. Changes over time in circulating antibodies, as assayed by hemagglutination (HA) and hemolytic reaction (HR) tests as well as counts of plaque forming cells (PFC) from the spleen were examined. Differences in the immune response were compared.The following results were obtained:1) The effects of 25°C and 18°C temperatures were quite similar, an ambient temperature of 15°C suppressed the immune response, suggesting that a critical point exists between 18°C and 15°C.2) Of the experimental temperatures, 4°C most conspicuously affected the activities of HA and HR as well as the number of PFC.3) However, when the ambient temperature was lower than 4°C, i.e. 2°C or 1°C, immune responses got nearer toward to the control level.4) Differences in the immune response based on the number of mice per cage were not obtained in either 2°C or 1°C ambient temperature.5) The influence of changes in temperature was greatest when the mice were transferred from 25°C to 4°C three days before immunization.
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