発熱性物質およびその除去に関する研究 (第1報) : 体温上昇変化パターンおよび Amoebocyte Lysate Test の検討

概要

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Detection of pyrogen in injection is generally performed as provided by official regulations. The procedure of the Pyrogen Test depends upon the measurement of body temperature rise until 3 hours after injection in rabbits and does not affect the temperature rising pattern.Comparetive investigations between bacterial pyrogens and hyperthermic chemicals were made by the observation of temperature rising pattern after injection. In addition, the method employing Amoebocyte lysate prepared by horseshoe crab was compared with the Pyrogen Test.Results obtained are summarized as follows:(1) Distinction between bacterial pyrogen and hyperthermic chemicals seemed to be possible by observing the rising temperature pattern.(2) Carmine known as one of hyperthermic chemicals does not seem to cause fever by chemical substance-Carmine itself, but it may be derived from bacterial pyrogen.(3) The pyrogenicity on rubber tube connected in disposable transfusion apparatuses seems to be derived from bacterial pyrogen.(4) With the rise of fever activity in various LPS, a difference of detection sensitivity between Pre-Gel Test and the Pyrogen Test showed a tendency to decrease. Further studies on detection sensitivity in various LPS extracted from various germs would be required to confirm the peculiarity of LPS and preparation of Amoebocyte lysate.
日本衛生学会の論文