脂溶性タール色素Tolylazo-naphthylamine (Oil Yellow OB)の毒性に関する実験的研究
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概要
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The present study was designed primarily in order to determine the effect of oral administration of Oil Yellow OB which had fed to rats at dietary levels of 0.75, and 0.5 per cent for three months and at 2.0, 1.0, 0.5, 0.25, and 0.1 per cent for two years. Single oral administrations was also done on mice.Throughout the study the animals were weighed weekly and physical conditions observed. Autopsies were performed on animals which died as well as which were sacrificed. Tissues were fixed for histopathologic examinations.Results were as follows:1. In a single oral administration of Oil Yellow OB, the total mortality of the mice that were given the maximum doses of 3.8gm. per kilo. was 40 per cent. The LD50 value was not obtainable due to its insolubility.Remarkable changes in liver were observed in all animals sacrificed after 10 days: the main changes observed were one or more of diffuse liver cell enlargement, excessive mitosis, and focal necrosis.2. Rats which were fed Oil Yellow OB at 0.75 and 0.5 per cent of the diet had a marked weight loss. In the liver, irregular arrangement and enlargement of the liver cells, sometimes accompanying mitosis, were observed in the early stages, followed by massive necrosis and collapse in the later atage.Thrombi were often observed in portal vessels.3. In long term feeding studies, rats which were fed Oil Yellow OB in over 0.25 per cent of the diet showed high mortality, appetite loss and marked growth inhibition, whereas the dose of 0.1 per cent level produced no appreciable effect except slight growth retardstion. In the liver, marked changes were observed in doses of more than 0.25 per cent, while there were slight changes at 0.1 per cent. The main change was centrolobular necrosis which was followed by fibrosis and cirrhosis. Nodular hyperplasia of liver cells was sometimes observed, and atypical proliferation was present in one rat which was fed at a 0.25 per cent level and sacrificed at the 53rd week.Changes were also present in the heart and kidney in some cases.
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