生体試料中微量重金属測定法の検討
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概要
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The purpose of this investigation was to establish a practical and accurate method for analyzing heavy metals in biological materials, particularly in edible animals.<BR>This paper describes a simple method of biological tissue sample preparation, the "Mild Digestion Method, M-D" which can be used for the analysis of various metals, and what is compared M-D with the conventional method, the "Complete Dogestion Method, C-D" which is sulfuric acid, nitric acid, perchloric acid digestion. The tissue was added to 2 to 2.5 times its own volume of concentrated nitric acid and heated at a relatively low temperature (95-100°C), overnight. The product was directly used as a specimen for flame-AAS and non-flameAAS without any further chemical treatment. The following results were obtained:<BR>1) The method was simple enough to prevent errors by contamination during the pretreatment process.<BR>2) Only a small amount of nitric acid was required for digestion when an adequate apparatus and heating temperature were selected. The final volume was adequate for analysis. Additionally, the nitric acid inhibited the interference of the large amounts of sodium and potassium which existed in the biological materials.<BR>3) Using this method, the measurement of metals at the ppm level was achieved by flame-AAS, and at the ppb level by non-flameAAS. The method, however, was inadequate for the analysis of arsenic without further chemical treatment.<BR>4) The respective recovery (%) and the detection limits (ng/ml) by non-flameAAS were as follows: Cadmium 101 and 0.02, Lead 98 and 0.71, Manganese 108 and 0.21, Nickel 108 and 0.34, and Chromium 106 and 0.50.<BR>The method was concluded to be simple, accurate and practically useful.
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