インフルエンザ・ワクチン接種により産生された抗鶏卵成分抗体の研究
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概要
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(I) Various techniques in agglutination methods using passively sensitized particles were compared for the purpose of determining a suitable test method for detecting small amounts of univalent antibodies toward soluble substances in chicken eggs.1) Univalent anti-egg albumin (-EA) antibody, produced in primary response immune sera of rabbits, proved its serological activity in all three agglutination tests of EA-sensitized particles, i.e., passive hemagglutination, bentonite agglutination and latex agglutination.2) A tube technique using latex particles sensitized using small amounts of egg materials was determined to be the method best suited for this study.3) Appropriate concentrations of EA, egg yolk (EY) and arantoic Fluid proteins (AF) to sensitize latex particles were determined.(II) Sera from influenza vaccine recipients and nonrecipients were tested by latex agglutination using particles sensitized with EA, EY and AF. Results obtained were as follows:1) In all 589 recipients' sera, 107 (18.17%) samples proved to contain one or more kinds of univalent antibodies toward chicken egg materials. Only 3 (1.17%) 237 non-recipients' showed positive reactions to the antibodies.2) Nonspecific latex agglutination occurred in the tests of 36 (6.11%) samples of sera from the recipients. In the remaining 553 samples, 48 (8.86%) proved anti-EA activity, 70 (12.66%) for anti* EY and 51 (9.22%) for anti-AF respectively. Relatively high incidence of anti-EY was estimated partially due to the nonspecific agglutination of EY-sensitized particles.3) Titers obtained by latex agglutination methods were lower than those by passive hemagglutination test. However, it was found that these two test methods showed similar rates of antibody detected sera due to the relatively low incidence of nonspecific latex agglutination.4) Antibodies against egg materials in recipients' and nonrecipients' sera revealed serological reactions by neither ring precipitin test nor two-dimensional immunodiffusion using cellulose acetate membrane.5) No statistical relationship was found between frequencies in production of anti-egg material antibodies and the classification of some blood groups, ABO, MN, P and Rh (D).6) No correlation was found between the presence in sera of anti-egg material antibodies and HI antibody titer toward influenza/Hongkong virus which was an ingredient of the vaccine.
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