AN <I>IN VIVO</I> ASSAY METHOD FOR CORTICOTROPHIN-RELEASING FACTOR (CRF) IN DEXAMETHASONE-NEMBUTAL TREATED RATS

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概要

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• The effect of dexamethasone on pituitary-adrenal function was studied in rats for the purpose of developing a new assay method for corticotrophin-releasing factor (CRF) in vivo. Intraperitoneal administration of 0.2mg of dexamethasone and 5.0mg of Nembutal per 100g body weight lowered the plasma corticosterone concentration in saline injected rats (3.5±0.3μg) and suppressed the effect of a wide variety of stressful stimuli under the level of 10.0μg/100m<I>l</I> of plasma. This treatment also inhibited the corticotrophic activity of vasopressin in doses less than 80 mU of activity. Partially purified CRF from the posterior pituitary was tested at several dose levels; a linear dose response curve was obtained between 0.04 to 2.2μg of CRF and plasma corticosterone level.<BR>It is proposed that the dexamethasone and Nembutal treatment is a new assay system for CRF, which is simple and suitable for routine assay procedure.<BR>The relationship between CRF and vasopressin and the blocking mechanism of dexamethasone are briefly discussed.

• 一般社団法人 日本内分泌学会の論文

著者

• ITOH ZEN

  First Department of Surgery and institute of Endocrinology, Gunma University School of Medicine

関連論文

• AN IN VIVO ASSAY METHOD FOR CORTICOTROPHIN-RELEASING FACTOR (CRF) IN DEXAMETHASONE-NEMBUTAL TREATED RATS

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