FURTHER OBSERVATION OF THE IMMUNOLOGIC DETERMINATION FOR HUMAN LUTEINIZING HORMONE

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1) Extracting 24hrs. urine by the kaolin-acetone method, immunologic and biologic assays were performed simultaneously for comparative study of luteinizing hormone (LH). Continuous daily measurements in normally menstruating women found theLH peak on time of ovulation; and an approximate agreement was observed betweenthe immunologic and biologic assay. 2) When measuring LH by the hemagglutinationinhibition technique, the end point is occasionally difficult to identify becausethe ring pattern appearing on the bottom of the test tube is atypical. We found that the use of Sephadex G 25 made every ring pattern distinct, almost completely eliminated the atypical ring, and enabled more accurate measuring than withoutthe use of Sephadex. 3) Serial LH variations. during one menstrual cycle ofeach subject showed a fairly good agreement with biologic assay. Immunologic and biologic assays in each urine collected at random showed comparatively parallel results in both assays. However, some cases showed lower values by immunologic than by biologic assay. The possible reasons inducing such dissociation in both assays are discussed.
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