Reaction Mechanism of Hexokinase Isozymes and Their Insulin Response

スポンサーリンク

概要

• 論文の詳細を見る

• Behavior of multiple hexokinase in the supernatant extracted from alloxan diabetic rat tissues after an insulin administration has been investigated by the DEAEcellulose chromatography. The activity of type II in diabetic liver and muscle, and the activity of type IV in diabetic liver markedly decreased, and by the insulin administration recovered, although the level of type II in muscle did not reach to normal in 24 hrs. after the injection. On the other hand, types I and III were not subject to control by insulin. Hexokinase type I and type II in liver were further purified by the DEAEcellulose chromatography, ammonium sulfate fractionation and hydroxylapatite chromatography, and kinetics and reaction mechanism were studied with these purified enzymes. The initial rate experiments indicated that the insulin insensitive type I showed a different reaction mechanism from the insulin-sensitive type II. The reaction of liver hexokinase type I appears like a ping-pong pathway in which one or more products are released, before all the substrates are added to the enzyme. Type II will be a sequential where ATP and glucose must bind to enzyme before any products are released.

• 社団法人 日本内分泌学会の論文

著者

• MACHIYA TADAHIKO

  Nutritional Laboratory, School of Health Sciences, Faculty of Medicine, University of Tokyo

• HOSOYA NORIMASA

  Nutritional Laboratory, School of Health Sciences, Faculty of Medicine, University of Tokyo

関連論文

• Response of “Key Glycolytic Enzymes” to Insulin
• Reaction Mechanism of Hexokinase Isozymes and Their Insulin Response

スポンサーリンク