Isolation of Thyroidal Acid Protease and Immunological Analysis of its Antibody

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概要

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• A method is presented for deriving a highly purified acid protease from calf thyroid. Thyroglobulin hydrolysis was very slow at pH 3.5 in comparison to hemoglobin hydrolysis. Protease was purified 285-fold from thyroid supernatant fraction by DEAE<SUP>+</SUP>Cellulose and Sephadex G-200 chromatography. The protein sedimented as a single component in the analytical centrifuge with a sedimentation constant of 3.6 S. Enzyme activity of the purified enzyme was increased by mercaptoethanol and cyste ne, and inhibited by n-chloromercuribenzoate.<BR>A specific antibody against purifisd human-acid protease was produced in rabbits.<BR>In the sera of patients with various thyroid diseases, antibody titers against acid protease were examined. No positive antibody titer was observed. The relation between the <I>auto-immune</I> disease in thyroid and antibody formation to acid protease is discussed.

• 社団法人 日本内分泌学会の論文

著者

• DEGROOT Leslie

  Thyroid Study Unit, The University of Chicago

• Ochi Yukio

  Department Of Laboratory Medicine Shiga University Of Medical Science

• DEGROOT LESLIE

  Thyroid Study Unit, Department of Medicine, University of Chicago Chicago

• OCHI YUKIO

  Department of Internal Medicine, Kyoto Prefectural University of Medicine

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