Heptafluorobutylation of Catecholamines and its Application to Gas-Liquid Chromatographic Analysis by Electron Capture Detector

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A method for formation of heptafluorobutyl derivatives of catecholamines and its use to gas-liquid chromatographic (GLC) analysis by electron capture detector (ECD) have been studied. When 0.01ml of heptafluorobutyric anhydride is used for the reaction to catecholamines in 0.1ml of ethyl acetate at 60°C for 30min, formations of heptafluorobutyl derivatives occur for all hydroxyl and amino groups of them. Heptafluorobutyl derivatives of catcholamines have good GLC properties and are more sensitive to ECD than other derivatives of them, i.e. trifluoroacetyl or trimethylsilyl ether-heptafluorobutyl derivatives. Separations of epinephrine, dopamine and norepinephrine are almost satisfactory, when 2% XF-1105 are used as stationary phase at 170°C. It has been recognized that heptafluorobutyl derivatives are applied to GLC analysis of catecholamines in biological fluids.
社団法人日本内分泌学会の論文