A Radioimmunoassay of Porcine Proinsulin C-Peptide Immunoreactivity

概要

論文の詳細を見る
Antiserum to porcine proinsulin was prepared in a guinea pig. Removal of crossreacting insulin antibodies was achieved by addition of excess porcine insulin. After such treatment, antiserum retained reactivity to porcine proinsulin but lost its ability to bind insulin. Displacement of labeled porcine proinsulin was not observed with 50ng or less of insulin. Both of the synthetic porcine proinsulin C-peptide analogue (62-formyllysine porcine proinsulin sequence 31-63) and the extracted porcine proinsulin C-peptide analogue (sequence 33-63) reacted approximately as well as porcine proinsulin on a molar basis. Cross-reactions between porcine glucagon and bovine proinsulin were not evident. Almost 100per cent recovery of added porcine proinsulin was noted from talctreated porcine sera. The coefficient of variance was 6.5per cent at a level 10 times the sensitivity for the two assays, and maximum sensitivity of the assay was 20 picogram. Assayed values were expressed as C-peptide immunoreactivity (CPR), since porcine proinsulin and porcine proinsulin C-peptide exist in peripheral blood, and moreover, porcine proinsulin intermediate forms might be exisist in peripheral blood. Immunoreactivity of porcine proinsulin in unextracted peripheral blood was assayed in 3 pigs after intravenous glucose load as CPR. Plasma CPR levels at fasting, 30min, and 60min after glucose load were from less than 0.2 to 0.4, 1.65, and less than 0.2 to 0.98ng per ml, respectively. A specific radioimmunoassay of porcine CPR in unextracted peripheral plasma has been described.
社団法人日本内分泌学会の論文

A Radioimmunoassay of Porcine Proinsulin C-Peptide Immunoreactivity

スポンサーリンク

概要

著者

関連論文

スポンサーリンク