Effect of Temperature during Preparation of Rat Adrenal Quarters and Isolated Cell Suspension on Their ACTH Responsiveness

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The effect of temperature during preparation of rat adrenal quarters or isolated adrenal cell suspension on their response to ACTH was examined through a comparison of amounts of corticosterone produced after their incubation.<BR>The response to ACTH added <I>in vitro</I> was considerably higher when adrenal quarters and isolated adrenal cell suspension were prepared at room temperature (25°C) than when prepared at ice-cold. Endogenous steroidogenesis was not affected by the temperature.<BR>It seemed unlikely that this higher response to ACTH of adrenal quarters or isolated adrenal cell suspension prepared at room temperature was due to an activation of the cells. A possibility was discussed that cooling adrenal quarters or isolated adrenal cell suspension during the prepara
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