Specific Binding of <SUP>125</SUP>I-Labeled Human Follicle Stimulating Hormone to Testicular Slice

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概要

• 論文の詳細を見る

• A simple radioreceptor-assay technique for human follicle stimulating hormone (hFSH) with use of rat testicular slice and enzymatically <SUP>125</SUP>I-labeled hFSH was described. It was shown that 125I-hFSH bound to the testicular slice (30mg) was approximately 2% of the total amount added to the tube (2ng/tube), half of which was displaceable in the presence of 1μg/tube of unlabeled hFSH. Although only small amount of <SUP>125</SUP>I-hFSH was bound specifically to the tissue, the binding was not inhibited by other peptide hormones tested. The testicular receptor for hFSH had high affinity constant (2.5×10<SUP>9</SUP>M<SUP>-1</SUP>) and hormonal specificity, suggesting a possibility of the physiological application of this radioreceptor assay for the measurement of hFSH in body fluids.

• 社団法人 日本内分泌学会の論文

著者

• MIYACHI YUKITAKA

  The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo

• INOMATA MITSUSHI

  Tokyo Metropolitan Institute of Gerontology

関連論文

• Enzymatic Radioiodination of Succinyl Cyclic AMP Tyrosine Methyl Ester by Lactoperoxidase and Radioimmunoassay for Cyclic AMP
• Specific Binding of 125I-Labeled Human Follicle Stimulating Hormone to Testicular Slice

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