A Receptor Site for Prolactin in Lactating Mouse Mammary Tissues
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概要
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Prolactin iodinated by lactoperoxidase method showed immunologically, electrophoretically and biologically similar properties to native prolactin and possessed enough specific radioactivity for receptor studies.<SUP>125</SUP>I-prolactin was incubated with mouse mammary tissues at 8 days of lactation. Both binding and release of<SUP>125</SUP>Iprolactin depended on incubation time and temperature and were maximal at 37°C. Michaelis constant was estimated to be 1.4×10<SUP>-9</SUP> M from Lineweaver-Burk plot and to be 1.2×10<SUP>-9</SUP> M from mid-value of the dose-response curve for displacement with native prolactin. Total number of binding sites for prolactin was 1.38×10<SUP>-15</SUP> mole per mg weight of tissue. Ovine prolactin, human growth hormone and human placental lactogen competed with <SUP>125</SUP>I-prolactin and dose-response curves for these three hormones were all parallel. These results suggest the existence of a specific receptor site with high affinity for prolactin in lactating mouse mammary glands.
- 社団法人 日本内分泌学会の論文
著者
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Sakai Senkiti
Department Of Animal Breeding Faculty Of Agriculture University Of Tokyo
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Kohmoto Kaoru
Department Of Animal Breeding Faculty Of Agriculture University Of Tokyo
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JOHKE TETSU
Department of Physiology, National Institute of Animal Industry
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