Free C-peptide immunoreactivity in insulin-treated diabetics.

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The serum samples of healthy and insulin-treated subjects were gel-filtrated before and after acid-alcohol extraction. The sera from insulin-treated diabetics were revealed to contain a variable amount of proinsulin-like components (PLC) which were compounded with insulin antibodies and accumulated in the circulation. These PLC-insulin antibody complexes were found to cross-react with C-peptide antiserum. In this study, we have determined serum C-peptide immunoreactivity (CPR) before and after the removal of PLC-insulin antibody complexes, and expressed as total CPR and free CPR, respectively.<BR>The separation of PLC-insulin antibody complexes was performed by the addition of polyethylene glycol to the serum. The free and total CPR values in the subjects never on insulin (7 healthy subjects and 25 adult onset diabetics) were correlated well with each other. The ratios free CPR: total CPR (F/T) were 1.02±0.17 (fasting) and 1.00±0.08 (60min after glucose). In contrast, F/T in insulin-treated diabetics (adult onset 29 cases) were apparently lower than that of the former group ; 0.58± 0.23 (fasting), 0.67±0.22 (60min after glucose). These results provide further evidence that the total CPR values were reflected to a considerable extent by the PLC-insulin antibody complexes. Some cases of insulin-treated diabetics reavealed that over 50% of their serum CPR was attributed to PLC-insulin antibody complexes, and this may be responsible for the higher CPR levels of these cases.<BR>Determination of free CPR may provide more accurate index to evaluate the endogenous insulin responses to various stimuli in some of the insulin-treated diabetics.
社団法人日本内分泌学会の論文

Free C-peptide immunoreactivity in insulin-treated diabetics.

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