Effects of Parathyroid Hormone and Prostaglandin E<SUB>1</SUB> <I>in vitro</I> on Release of Cyclic AMP from Kidney Cortical Tissue

スポンサーリンク

概要

• 論文の詳細を見る

• The interaction of parathyroid hormone-(PTH) and prostaglandin E<SUB>1</SUB> (PGE<SUB>1</SUB>) on cyclic AMP metabolism in the rat renal cortical tissue was examined <I>in vitro</I>, placing an emphasis on the process of release of the nucleotide from the tissue. PTH enhanced the release of cyclic AMP from cells even in the presence of 10μM cyclic AMP added to the incubation medium. This effect of PTH to accelerate cyclic AMP release was suppressed by simultaneous addition of PGE<SUB>1</SUB>. Although PTH and PGE<SUB>1</SUB> showed an additive effect in stimulating the adenylate cyclase activity of the purified renal plasma membrane, in the experiment in which slices were employed the sum of cyclic AMP in the tissue and medium under simultaneous stimulation tended to be less than that under the stimulation by PTH alone. However, the increase by PTH of specific activity of cyclic AMP produced in slices prelabeled with [<SUP>14</SUP>C] adenine was further enhanced by the simultaneous addition of PGE<SUB>1</SUB>, suggesting that PGE1, by suppressing the release of cyclic AMP, keeps more cyclic AMP intracellularly and offers phosphodiesterase more substrate. It seemed that the release of cyclic AMP from the kidney cortical tissue could proceed against a concentration gradient and was regulated somehow by PTH and PGE<SUB>1</SUB>. It was noted also that the change in the release process influenced the intracellular metabolism of cyclic AMP, indicating the importance of taking such an extracellular release mechanism into consideration in the study on the cellular metabolism of cyclic AMP.

• 社団法人 日本内分泌学会の論文

著者

• NAGATA NAOKAZU

  Clinical Division, Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology

• KIMURA NARIMICHI

  Clinical Division, Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology

• ARAKI NOBUKO

  Clinical Division, Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology

関連論文

• Effects of Adrenergic and Cholinergic Stimulations on Cyclic Nucleotides Metabolism in Bone of Young Rat
• Effects of Parathyroid Hormone and Prostaglandin E1 in vitro on Release of Cyclic AMP from Kidney Cortical Tissue

スポンサーリンク