Binding Protein of Somatomedin A in Serum from Men and Rats

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After gel chromatography of human and rat serum at pH7.4, all endogenous somatomedin A was recovered in the high molecular weight range. The largest peak was found in the γ-globulin (II) region and the next largest peak found in the albumin region (III). The amounts of somatomedin A in the peak II region increased in serum from acromegalies and decreased in serum from growth hormone deficient patients.<BR>Four radioactive peaks were observed after gel chromatography of serum incubated with <SUP>125</SUP>I-somatomedin A. Only the two peaks corresponding to peaks II and III out of the four peaks were displaced by adding 50μg of partially purified cold somatomedin A. The radioactivity of peak II decreased in sera from growth hormone deficient patients and increased after growth hormone administration.<BR>These observations support the hypothesis that the growth hormone regulates not only somatomedin A but also its carrier protein.
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