Preovulatory Changes in Luteinizing Hormone Releasing Hormone Concentrations in Peripheral Plasma in Constant Estrous Rats at Induced Ovulation
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概要
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Cervical stimulation was used to induce synchronous ovulation in rats that were induced into constant estrus by exposure to continuous lighting. Anti-LHRH or anti-LH serum completely blocked the induced ovulation when administered iv within 5 or 40min after cervical stimulation. Peripheral concentrations of LH and LHRH hypothalamic content of LHRH were quantified before and at various intervals (0.5-70min) after cervical stimulation. The radioimmunoassay for LHRH employed a specific antiserum, OAL-513, with high affinity and specificity and highly purified <SUP>125</SUP>I-LHRH. Measurement of LHRH in extracts of 3-5ml of plasma gave values that were readable in the middle portion of the standard curve.<BR>A two-fold increase in the LHRH concentration in peripheral plasma was observed 30sec after the start of cervical stimulation. The elevated LHRH level was sustained for 15min, though associated with large fluctuations. Serum LH levels started to rise 4 to 10 min and returned to the basal level 70min after cervical stimulation. The hypothalamic LHRH content continued to increase throughout the entire experimental period and attained 140% of the initial level. No significant deflection due to LHRH release was observed in the hypothalamic LHRH content. Changes in the peripheral hormone level after cervical stimulation were competent for chronologically reasoning the effectiveness of the antihormone treatment for blockage of the induced ovulation.<BR>From these results it is concluded that LHRH release before the preovulatory LH surge in light-estrous rats was brief in duration and erratic in mode.
- 社団法人 日本内分泌学会の論文
著者
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TAKAHASHI MICHIO
Laboratory of Veterinary Physiology, Faculty of Agriculture, University of Tokyo
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HASHIMURA ETSURO
Research Division, Otsuka Assay Laboratory, Otsuka Pharmaceutical Co., Ltd.
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SHIN SADAHITO
Research Division, Otsuka Assay Laboratory, Otsuka Pharmaceutical Co., Ltd.
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IMAGAWA KENICHI
Research Division, Otsuka Assay Laboratory, Otsuka Pharmaceutical Co., Ltd.
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SUZUKI YOSHISUKE
Laboratory of Veterinary Physiology, Faculty of Agriculture, University of Tokyo
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TAKAHASHI Michio
Laboratories of Veterinary Physiology
関連論文
- Changes in Concentration of Hypothalamic Cytosolic Progestin Receptors at the Initiation of Pseudopregnancy in Rats
- Preovulatory Changes in Luteinizing Hormone Releasing Hormone Concentrations in Peripheral Plasma in Constant Estrous Rats at Induced Ovulation
- Light-and-Dark Signal for the Initiation of Prolactin Surges in Cervically Stimulated Rats
- Ovulation in Light-Estrous Rats Induced by Darkness
- Production of Anti-Glucagon Sera with A C-Terminal Fragment of Pancreatic Glucagon
- Changes in 20.ALPHA.-Hydroxysteroid Dehydrogenase mRNA in the Ovary of Immature Rats Bearing a Single Generation of Corpora Lutea.