Further Evidence that Gonadal Steroids do not Modulate Brain Opiate Receptors in Male Rats

概要

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It is still unclear whether, in the male rat, castration and androgen replacement affect the binding characteristics of brain opiate receptors. To clarify this issue, the effects exerted by orchidectomy and testosterone (T) replacement on the subpopulation of brain μ opiate receptors were studied in male rats; testosterone was administered via subcutaneous Silastic capsules. Utilizing <SUP>3</SUP>H-dihydromorphine (a μ receptor ligand) it has been shown that the affinity constant (Ka) of brain μ opiate binding sites, measured in plasma membrane preparations, is not affected by castration. When μ receptor concentrations were measured in individual brains, it was found that gonadectomy and T replacement failed to produce any change in the number of μ opiate receptors. These data suggest that, in male rats, gonadal steroids do not develop their central feedback effects by affecting brain μ opiate receptors.