ポーラログラフ法によるメチオニンの定量法
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概要
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Methionine is one of the polarographically inactive substances and its oxidized products, methionine sulfoxide and methionine sulfone, are also as inactive as methionine. Consequently, it was thought impossible to obtain polarographic reduction wave directly from methionine or its derivatives. However, the attention had been paid to the fact that PbO<SUB>2</SUB> added to methionine as an oxidant was reduced quantitatively to Pb<SUP>2+</SUP> and the polarographic determination of lead ion thus produced was found to be possible to determine the methionine indirectly. The recommended procedure is as follows: One <I>ml</I> sample solution containing about 0.01<I>mM</I> methionine is taken in 20<I>ml</I> measuring flask, about 20mg of PbO<SUB>2</SUB> and 1<I>ml</I> 7<I>N</I> HNO<SUB>3</SUB> are added, shaken for 30?40 minutes at room temperature, after the reaction is over, 1<I>ml</I> 3<I>N</I> HNO<SUB>3</SUB> and 0.4<I>ml</I> 0.5% gelatin solution are added and the solution is made up to 20<I>ml</I> with distilled water, the solution is filtered, polarogram of the filtrate is recorded by the usual method and the methionine is determined by the wave of substitutedlead ion.
- 社団法人 日本分析化学会の論文