X線顕微鏡による生物試料の観察

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Several methods in the development of X-ray microscopy were tested, including the imaging method with zone plates and grazing-incidence mirrors. With zone plates, spatial resolution of 0.5 μm was attained. With grazing incidence mirrors, the spatial resolution of 0.2 μm was achieved. With both methods, zone plates, diatoms, muscle, collagen and other biological specimen were observed.<BR>As a compact X-ray source, AURORA seems to be promising. We have tested contact X-ray microscopy of Hela cell and chromosomes. With 10 s exposure (ring current-2 mA), good contrast image was taken.<BR>Spatial resolution was about several tens nm. Points to be improved were discussed for each method.
社団法人日本分光学会の論文