分配クロマトグラフィーによるコハク酸と乳酸の分離
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概要
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The mixture of succinic and lactic acids has been hardly isolated even with a longer column. In practice, in order to transfer the sample solution containing small amounts of the acids quantitatively onto a silica column (55cm long, 0.7cm dia.), the solution is mixed with one gram of silica gel which gives 6 cm of the sample column. The two acids are completely separated. by the development with an 18% <I>tert</I>-butanol-chloroform accompanied by a preliminary development with 10m<I>l</I> of 14% <I>tert</I>-butanol-chloroform. The effect of the preliminary development to give the clear-cut separation of the elution bands is attributed to the concentration gradient of <I>tert</I>-butanol occurring around each solvent front, namely, this concentration gradient is effective in constricting the individual chromatograms in the column.
- 社団法人 日本分析化学会の論文