薄層クロマトグラフィーによるピリジンカルボン酸の検出
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概要
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Chromatograms were developed in 3 to 4 hours by an ascending technique with a solvent mixture of <I>n</I>-butyl alcohol-acetic acid-water (4:1:1, v/v). Chromatograms developed were dried and sprayed with the reagent of following compositions: 1% aqueous solution of Mohrs salt, 10% aqueous solutions of NH<SUB>2</SUB>OH·HCl and pyridine (20:2:1, v/v) for the identification of pyridinecarboxylic acid whose carboxylic group being located at the α-position and then sprayed with a 2% alcoholic solution of 2, 4-dinitrofluorobenzene for the detection of nicotinic acid and isonicotinic acid. The identification limit was found to be 2 μg of picolic acid and nicotinic acid, and 4 to 6 μg for other pyridinecarboxylic acids. R<SUB>f</SUB> values of pyridinecarboxylic acids obtained are in the following order: pyridinemonocarboxylic acids>pyridinedicarboxylic acids>pyridinetricarboxylic acids.
- 社団法人 日本分析化学会の論文