チロシンおよびトリプトファンの特異呈色反応

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概要

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• Tyrosine and tryptophan were spotted on thin layer of Japanese acid clay and the plate was air-dried at room temperature.<BR>Ninhydrin solution(0.5% ninhydrin in 95% ethanol) was sprayed on that plate, which then was heated at 100110°C for 10 min.<BR>After cooling, a blue or dark green stain appeared within 12 hours.<BR>The blue stain of tyrosine turned red by a drop of 3<I>N</I> NH<SUB>4</SUB>OH, and this turned blue by a drop of 3<I>N</I> HCl. On the other hand, the color of tryptophan did not changed by NH<SUB>3</SUB> and hence by HCl. The tests were not interfered from other amino acids.

• 社団法人 日本分析化学会の論文

著者

• 横山 香寿子

  岐阜大学教育学部化学教室

関連論文

• チロシンおよびトリプトファンの特異呈色反応

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