血清中総ビリルビンの迅速比色定量
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概要
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A diazo reagent prepared by diazotization of sulfanilamide was applied to the rapid determination of total bilirubin in serum as azobilirubin with colorimetry, and the condition of determination was optimized.<BR>The absorption maximum of the colored solution obtained by the recommended standard procedure was at 545mμ. The method has advantages in faster color development and higher absorbance than Evelyn-Malloy's method, which uses the diazo reagent prepared by diazotization of sulfanilic acid and is now in general use in clinical laboratories.
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