吸光度比法における定量の精度および正確性
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概要
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In the previous paper, the absorbance ratio method was developed and was applied to quantitative determination of various drugs, reagents and food additives.<BR>In this paper, factors influencing on the precision and accuracy of determination in this method were investigated to keep the deviation of the assay values within 0.1%. As the results, it was found that, as for the absorbance measurements(Table I), |d<I>r</I>/d<I>x</I>|value, differentiation of the absorbance ratio by the reciprocal of neutralization grade, must be more than 35, and as for the addition of volumetric standard solution (Table II), the determination is carried out precisely in case a whole pipet is used. In the determination of quinine ethylcarbonate and aminopyrine using this method, the influences of wavelength(Table III), of indicator lot (Tables VIII and XIII), of indicator concentration (Table IX), of sample concentration (Table X)and of time exposed (Table XI) on the assay value are negligible, and the influence of solvent concentration (Tables IV and V)is also negligible provided the solvent concentration is exactly adjusted. The lnfluence of temperature (Tables VI, VII and XII and Fig.1) is also negligible in case suitable range of <I>x</I> values is used. lf the assay value is calibrated for temperature by formula (1) and Fig. 1, the other range of <I>x</I> values can also be used. The similar results were obtained on the other substances tested.<BR>It was confirmed from the results of several investigations (Tables XIV, XV and XVI)that the <I>x</I>-<I>r</I> working curves are accurately prepared.<BR>As reported in the previous paper, the standard deviation (<I>n</I>=6) for all substances tested were within 0.1%<SUP>2)</SUP>.
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