セファロスポリンCの定量
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概要
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Cephalosporin C was treated with perchloric acid solution of 2, 4-dinitrophenyl hydrazine in a boiling water bath to form glyoxal bis 2, 4-dinitrophenyl hydrazone.<BR>Glyoxal bis 2, 4-dinitrophenyl hydrazone dissolved in dimethylformamide gave blue color by making pH above 12.5 with tetramethylammonium hydroxide. <BR>The absorbance measured at 615 mμ against dimethylformamide obeyed Beer's law from 2×10<SUP>-8</SUP> to 2×10<SUP>-7</SUP> mol/m<I>l</I> of Cephalosporin C. <BR>This method has been scrutinized to be applied to the determination of synthetic cephalosporins and penicillins.
- 社団法人 日本分析化学会の論文