血液中一酸化炭素ヘモグロビンの測定法
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概要
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A gas chromatographic method for the determination of CO-hemoglobin (CO-Hb) in human blood was investigated. A simple attachment for gas chromatograph, blood gas sampler (BGS-1A), in which CO was freed from CO-Hb with Van-Slyke reagent and directly transferred into gas chromatograph, was constructed. Performance of this BGS-1A, and analytical conditions were studied.<BR>Co was released from CO-Hb and analysed by following conditions; blood sample was 20 μ<I>l</I>, releasing reagent, 0.5 m<I>l</I>, reaction time in BGS-1A, 5 minutes, sampling time for GC, 30 sec, respectively. The setting of these sampling and reaction time must be kept precisely.<BR>Blood clotting disturb the release of CO from samples and the determination should be started as soon as blood samples were taken out. If the samples are required to be stored over 200 hours, the samples must be kept in refregerators.<BR>The content of CO-Hb in blood was calibrated from the saturated CO-Hb obtained by passing CO gas, or town gas through the blood for about 10 minutes.<BR>A digital integrator is useful for prosessing gas chromatographic data, and this CO-Hb analysing system easily gave the content of CO-Hb in micro amounts of blood samples. (20 to 50 μ<I>l</I>). The coefficient of variance of this method was calculated to be 0.024 when blood samples were taken 20 to 50 μ<I>l</I>.<BR>The higher level of CO-Hb in blood were obtained from the people, who were exposed in air pollution and smoking. It is interest that the smoking is one of the main factors to give higher content of blood CO-Hb.<BR>In this study, it was proved that this gas chromatographic analysis of CO-Hb were superior to conventional Van-Slyke method and useful for the CO determination in micro amounts of blood samples.
- 社団法人 日本分析化学会の論文