Drosophila GTPase Nucleostemin 2 Changes Cellular Distribution during Larval Development and the GTP-Binding Motif Is Essential to Nucleoplasmic Localization
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概要
- 論文の詳細を見る
Nucleostemin (NS), a nucleolar guanosine triphosphate (GTP)-binding protein, plays significant roles in cell cycle progression and ribosomal biogenesis. Drosophila Nucleostemin 2 (NS2), a member of the Drosophila NS family, regulates early eye development and is essential to cell survival in vivo, but the underlying mechanisms have yet to be clarified. Biochemical analysis using the recombinant NS2 protein indicated that NS2 has GTPase activity. Immunohistochemistry revealed that NS2 changes in subcellular locus from the nucleolus to the nucleoplasm during larval development, and that a mutation in the ATP/GTP-binding site motif A (p-loop) prevents nuclear localization of NS2 and results in cytoplasmic distribution. Furthermore, downregulation of NS2 altered the rRNA proportions between the nucleus and the cytoplasm. These results suggest that NS2 at least requires GTP to import into the nucleoplasm.
- 社団法人 日本農芸化学会の論文
著者
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Matsumoto Shogo
Molecular Entomology And Baculovirology Laboratory Riken
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Matsumoto Shogo
Molecular Entomology Laboratory Riken Advanced Science Institute
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MATSUO Eriko
Molecular Entomology Laboratory, RIKEN Advanced Science Institute
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NAGAMINE Toshihiro
Molecular Entomology Laboratory, RIKEN Advanced Science Institute
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TSUNEIZUMI Kazuhide
Molecular Entomology Laboratory, RIKEN Advanced Science Institute
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