Preventive Effects and Mechanisms of Rhein on Renal Interstitial Fibrosis in Obstructive Nephropathy

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概要

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• Renal interstitial fibrosis is a common outcome of a variety of chronic renal diseases. Here we evaluated the therapeutic efficacy of rhein on renal interstitial fibrosis induced by unilateral ureteral obstruction (UUO) and investigated the potential mechanisms. Mice underwent UUO, followed by orally administrated rhein (150 mg/kg/d) or control vehicle. Renal interstitial injury and the degree of fibrosis were evaluated by pathological staining and Western blot. The possible mechanisms were studied by Western blot, indirect immune-fluorescence and enzyme-linked immunosorbent assay. Our results showed that rhein therapy markedly ameliorated renal interstitial fibrotic lesions, reduced α-smooth muscle actin (α-SMA) expression, attenuated deposition of fibronectin (FN). Rhein also suppressed transforming growth factor-β1 (TGF-β1) and its type I receptor expression in obstructed kidneys. In vitro, rhein abolished the α-SMA and fibronectin expression of rat kidney interstitial fibroblasts cells (NRK-49F) induced by TGF-β1. These observations strongly suggest that rhein is a potent inhibitor of renal interstitial fibrosis, and its therapeutic mechanism is, at least in part, blocking interstitial fibroblasts cells activation.

著者

• Yang Junwei

  Department Of Macromolecular Science And Key Laboratory Of Molecular Engineering Of Polymers (minist

• He Dongyuan

  First Clinical Medical College of Nanjing Medical University

• Lee Li

  First Clinical Medical College of Nanjing Medical University

• Wang Xiaoyun

  First Clinical Medical College of Nanjing Medical University

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• Preventive Effects and Mechanisms of Rhein on Renal Interstitial Fibrosis in Obstructive Nephropathy
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