固相合成による甘味タンパク質モネリンの構造活性相関
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概要
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Monellin, an intensely sweet protein, was isolated from the fruit of the West African plant, <I>Dioscoreophyllum cumminsii</I> Monellin consists of two noncovalently associated polypeptide chains, the A chain with 44 amino acid residues and the B chain with 50 residues. The tertiary structure has been determined, and the native conformation is essential for the sweet taste. Recently, the primary structure of monellin was unambiguously determined, and monellin was synthesized by the Fmoc solid-phase method. In an attempt to delineate the active site(s) of monellin, amino acid residues within potential active sites were replaced. Replacement of Cys<SUP>B41</SUP> by Ser led to a small change in sweetness. Replacement of Ile<SUP>B6</SUP>, Asp<SUP>B7</SUP> or Ile<SUP>B8</SUP> by different amino acids resulted in a complete or marked loss of sweetness. Replacement of Asp<SUP>A16</SUP> or Tyr<SUP>A13</SUP> by other amino acids did not reduce the substantial sweetness. Replacement of each Lys residue by L-2-aminohexanoic acid (L-norleucine) significantly diminished the sweetness potency, but did not completely remove the sweetness. Analysis of the synthetic analogs by circular dichroism (CD) showed that the major structural features of monellin were not significantly altered. These findings suggest that Ile<SUP>B6</SUP>, Asp<SUP>B7</SUP> and Ile<SUP>B8</SUP> are responsible for eliciting a sweet taste, and that one or more basic residues may also be important for eliciting a sweet taste. In the course of the investigation, the D-enantiomer of monellin was synthesized by the Fmoc solid-phase method, and crystallized by the hanging drop vapor diffusion method. The D-isomer was shown by CD to be the mirror image of the synthetic L-monellin, and was devoid of any sweetness (essentially tasteless). DL-Monellin was crystallized from a 1 : 1 mixture of the synthetic L- and D-monellin, and its CD spectrum did not show any absorption. DL-Monellin was 2000 times sweeter than sucrose on a weight basis (cf., monellin is 4000 times sweeter than sucrose) These results indicate that the crystals consist of the racemates of DL-monellin.
- 社団法人 有機合成化学協会の論文