アリザリン・コンプレクソンの経口投与によるヒラメ稚魚の耳石染色

概要

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Otolith staining by alizarin complexon (ALC) was investigated to develop a new mass-marking method using oral administration in juveniles of the Japanese flounder Paralichthys olivaceus. Fishes of about 50mm in total length (82 days after hatching) were accommodated in three 500l tanks, and were supplied artificial diets treated with ALC solutions (ALC diet) for 15 days. ALC concentrations were 100, 500, and 1, 000mg/kg diet. Fishes were sampled every 5 days during the 15 days of ALC diets and then every month. Otoliths (sagittae) were photomicroscopically observed under G-exited UV light. A clear fluorescent mark was found on the peripheral zone of otoliths dissected immediately after ALC diet treatment, irrespective of the concentration. Otoliths taken from fishes with 1, 000mg-15 days treatment were most clearly stained. Fluorescent marks were not found in ctenoid scales on the ocular body side of the same fish. the fluorescent marks on the otoliths were evidently retained for 119 days of post-treatment at all three concentrations. These results indicate that otolith marking by oral ALC administration is effective and practical as a mass-tagging method in juveniles of Japanese flounder.
公益社団法人日本水産学会の論文