ニジマスの耳石形成と炭酸脱水酵素:小襄の酵素活性およびin vitroにおける耳石へのカルシウム沈着

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The involvement of saccular carbonic anhydrase (CA) in otolith formation was studied in cultivated rainbow trout. CA activity was electrometrically determined in the sacculus, gill, and blood. In vitro calcium uptake by the otolith in sacculus-otolith preparations was also examined in the presence and absence of Diamox. The sacculus contained CA in a concentration of 95 EU per g-wet tissue after correction for blood contamination. This activity was completely inhibited by Diamox. The gill and the blood had much higher CA activities of 1391 EU per g and 1544 EU per ml, respectively. In organ culture, the otolith showed a calcium uptake of 15.7×10-3μg per mg-dry tissue after 48h of incubation. Diamox significantly inhibited this uptake, by 21%. These results were discussed in terms of the functional involvement of saccular CA in the carbonate formation of fish otoliths.
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