ウニの冷凍に関する研究-II : ウニおよび魚卵のカルボニルの分離・定量法の検討

概要

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On account of the necessity to extract or separate carbonyl compounds from sea urchin gonads and fish eggs without changing their properties, the gas liquid chromatographic figures of carbonyls in these eggs, obtained by the ordinary steam-distilling method, was examined. In this examination the N2 gas bubbling method, a method of clear trichloro-acetic acid solution after removal of the protein and the newly devised 80% ethyl-alcohol extracting method, were compared. In addition to the above a simultaneous determination method of total carbonyl and oxoacid in fish eggs was investigated. The results are as follows; 1) A fairly large amount of acetaldehyde, and a small amount of acetone and crotonaldehyde, were produced. On the other hand iso-valeraldehyde or 3-methyl butylaldehyde decreased during steam-distillation. 2) Acetaldehyde was also produced by heating under N2 aeration, but iso-valeraldehyde or 3-methyl butylaldehyde did not decrease. 3) A small amount of threonine, together with glucose, decomposed to acetaldehyde during steam-distillation. 4) Recoveries of total carbonyl and oxo-acid contents in fish eggs determined by the newly devised method, were 92% on the average. The total carbonyl and oxo-acid content of sea urchin gonads frozen at -10±3°C for three months, was about three times as much as the value of walleye pollock eggs and rainbow trout eggs stored under same conditions.
公益社団法人日本水産学会の論文