ON THE VITAMIN B12 METABOLISM OF BIVALVES-II:STABILITY OF RADIOACTIVE VITAMIN B12 IN TISSUES OF TAPES JAPONICA AND IN ARTIFICIAL SEA WATER

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In the preceding paper, radioactive B12 absprbed in tissues of the little neck clam (Tapes japonica) was calculated directly from measured radioactivity. This calculation, however, cannot be established if radioactive B12 is decomposed and the labeled part loses B12 activity. Therefore, the stability of radioactive B12 in tissues of the clam and in artificial sea water has been examined in the present work. 1) Extracts from body tissue of the clam which absorbed radioactive B12 were prepared and spotted on the paper for chromatography using sec. butanol-H2O solution as developer. The paper was cut into ten pieces or so, each 1cm wide, in parallel with the original line. Radioactivity and B12 activity on Euglena gracialis were measured for these pieces (Fig. 1). The results showed that 60Co detected in the chromatogram existed as a constituent of certain substance which had a biological activity similar to that of vitamin B12. The fact suggests that radioactive B12 absorbed by the clam was not decomposed or, at least, maintained the activity for Euglena gracialis (Table 1). 2) When radioactive B12 was added to artificial sea water and was kept at 15°-20°C under the shelter from the daylight for 15 days, the radioactivity and the B12 activity on Euglena gracialis were found maintaining the initial levels (Table 2).
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