食品防腐剤に関する研究-III : 抗生物質に対するProteus morganiiの耐性獲得について

概要

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Acquisition of resistance against chlortetracycline (CTC) and oxytetracycline (OTC) by Proteus morganii, the causative organism of allergy-like food poisoning, has been studied. In addition, the influence of these antibiotics upon the formation of histamine and the activities of l-histidine decarboxylase of the washed cell suspensions has been investigated with the resistant and the original sensitive strains. Results obtained may be summarized as follows: 1. Four strains of P. morganii were exposed to a constant dose of each drug, either a maximum tolerance level or 0.1 ppm. The latter dose is the maximum permissible residual of CTC in fish regulated by the Japanese Food Sanitation Law. After 60 successive subcultures were made in nutrient broth containing either antibiotic, the resistance increased 4 to 16-fold at a miximum tolerance level and 2 to 8-fold at 0.1 ppm. 2. A complete cross-resistance between CTC and OTC was observed. A slight crossresistance against chloramphenycol was recorded with the OTC or CTC-resistant organisms, while that against streptomycin was not appreciable. 3. No marked difference in the rate of formation of histamine was observed between resistant and sensitive organisms when grown in the mackerel infusion. 4. Ten or 100 ppm of either drug did not interfere the activity of l-histidine decarboxylase of the washed cell suspensions of both resistant and sensitive organisms.
公益社団法人日本水産学会の論文