フグ毒定量法
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概要
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In the light of similarity of pfuffer poison to paralytic shellfish poison, we attempted to assay the former by the method adopted by H. Sommer and others in the estimation of the latter, hoping to obtain more accurate results with a smaller number of mice. The dose-effect curve of pfuffer poison shows to be just the same as that of the paralytic shellfish poison (Fig. I), suggesting the similarity of these poisons. The extraction of poison by methanol acidulated with hydrochloric acid, which has been recommended by the American workers in the studies of paralytic shellfish poison proves to destroy the pfuffer poison exceedingly. Methanol, acidulated with acetic acid (ca. pH 5), is successfully employed without this defect.
- 社団法人 日本水産学会の論文