Preservative Experiment of Smoked Shark-Fillet:Part III Production of ammonia

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In the present paper is discussed the production of ammonia in the smoking of Abura-zame-fillet during preservation. Ammonia was determined by Nessler's colorimetric method after being extracted the sample with acetic acid and the filtrate discolourized with active-carbon. The analytical data were shown in Tab. 1. In order to accertain the cause of ammonia production in the smoking, whether it is by autolysing or by bacterial action, experiments were made and the results were shown in Fig. 1. (A) and (B). From Fig. 1., we know bacterial action does more important role for the production of ammonia in the smoked muscle. The relation between the ammonia development and the relative humidity in which the samples were stored, are as shown in Fig. 2.
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