A New Method for Preparation of Sardine Scales

概要

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In this new method, fish scales are attached with their underside down. and without any fixative on ordinary microscope slide. Befor mounting the scales are cleaned off of their adherent matters and softened 15 seconds at 25-29°C in airtemperature immersing in acetocarmine solution being of 40% of acetic acid and saturated with carmine. The scales softened enough are picked up and placed on the slide taking care to avoid any air left between them, and excessive fluid is taken out. After these procedures the slides are gently dried off in room, and the scales are thus firmly fixed up for further examinations. It should be mentioned herein that the scales handled by the present method show little modification, and stretching (less than 1% in most caces as shown in the outermost column of Table 1) negligible for the purpose of further scrutinies. The method here recorded is successful on both scales, fresh and fixed by formarin, and is, at least, useful for the scales of Sardinia melanosticla, Engraulis japonicus, Etrumeus micropus, Spratclloides japonicus, Clupea pallasii, Harengula zunasi, and Cololabis saira, but useless for ones of Hemibarbus barbus and Acanthogobius flavimanus.
社団法人日本水産学会の論文