微生物によるアルギン酸の分解-VI : 2種のアルギナーゼの作用様式について

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概要

• 論文の詳細を見る

• Two alginase preparations, extracellular enzyme (ECE) and intracellular enzyme (ICE), have been obtained from filtrates of cultures and sonicated cells of an alginolytic marine bacterium. Both the enzymes cleave alginic acid into the same ultraviolet absorbing product, which indicates that the glycosidic bond of alginic acid is split by eliminative hydrolysis, resulting in the formation of unsaturated uronic acid moieties. However, marked differences in their modes of action have been recognized with aid of paper chromatographic detection. ECE attacks alginic acid in a random manner, liberating unsaturated oligouronides as well as a large amount of low polyuronide. The end products of this reaction are found to be a series of unsaturated oligouronides, which are not degraded further by this enzyme. In contrast, ICE attacks not only alginic acid but also its degraded products by ECE action. ICE is able to hydrolyze low polyuronide and such unsaturated oligouronides as tetra-, tri-, and diuronide. The ultimate end product of these reactions is found to be a monouronide, which seems to be identical with 4-deoxy-5-ketohexuronic acid reported by PREISS and ASHWELL.

• 公益社団法人 日本水産学会の論文

著者

• 安藤 芳明

  北海道衛生研究所

• 井上 勝弘

  北海道衛生研究所

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• 微生物によるアルギン酸の分解-VI : 2種のアルギナーゼの作用様式について
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