Rettgerella様菌を接種したタコ筋肉に生成されるマウスに対する有毒物質の協同因子の分離と同定

概要

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As shown in the previous paper1), a toxic substance having lethal toxicity to mice intraperitoneally has been proven to produce by a strain of Rettgerella-like bacteria in the octopus muscle extract medium, and the substance seemed to have a non-basic nature according to the phosphotungstic acid fractionation procedure. The basic fraction itself was not poisonous but enhanced the toxicity of the non-basic fraction. Present paper deals with the isolation and identification of the synergetic factor in the basic fraction. Results obtained may be summarized as follows: The synergetic substance was successfully isolated by the ion exchange resin technique, using an Amberlite IRA-400 (OH type) column for the lst step, and an Amberlite IRC-50 (H type) column for the 2nd step. The purified crystal was found to enhance the toxicity of the non-basic fraction (Table 1), and the phenomenon seemed to coincide with that reported in the previous paper1). This substance was identified as glycine-betaine from its Rf value on paper chromatograms (Fig. 1), and from its melting point of picrate derivative (Table 2).
社団法人日本水産学会の論文