Inhibitory effect of tropomyosin on Chara myosin motility

概要

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Chara myosin is a plant myosin involved in cytoplasmic streaming of Characean cells and the fastest of all myosins observed so far with in vitro motility assay. Movement of F-actin on Chara myosin was completely inhibited by muscle tropomyosin. In concert with this, the actin activated Chara myosin ATPase was also completely inhibited by tropomyosin. The movement of the thin filament of F-actin with native tropomyosin, the complex of tropomyosin and troponin, was inhibited on Chara myosin and the actin activated ATPase of Chara myosin was also inhibited by native tropomyosin. With careful observations, Chara myosin occasionally moved actin filaments in the presence of native tropomyosin and Ca2+. This indicates that the effect of tropomyosin of the thin filament in the“ON”state is not exactly the same as that of tropomyosin alone on F-actin.
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