A Combined Method of Immunocytochemistry of Choline-acetyltransferase Detection and of Autoradiography of (3H)choline after Retrograde Axonal Transport. Study of Cholinergic Pathways in the Central Nervous System at Light and Electron Microscopic Level.:S

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概要

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• Immunocytochemistry of choline-acetyltransferase (ChAT) has been combined with autoradiography of [3H]choline after retrograde axonal transport on the same tissue section. This combination was performed with efficient preservation, in situ, of [3H]choline or its derivatives by ionic fixation with silicotungstic acid. The cholinergic neuronal pathway from the diagonal band of Broca (DBB) of basal forebrain to the olfactory bulb (OB) was investigated with this method. After the injection of [3H]choline into the OB, radiolabelled neurons were identified in the DBB. Most of them were ChAT immunoreactive confirming the specificity of [3H]choline retrograde labelling for cholinegic neurons. At the electron microscopic level, silver grains could be observed only on the cytoplasm. This method will be useful to study the coexistence of transmitters in the cholinergic pathways and at the same time to determine the chemical nature of the afferences making contact with a defined soma of a cholinergic pathway.

• 日本学士院の論文

著者

• TSUJI Shigeru

  Laboratory for Neural Architecture

• MOTELICA-HEINO Ion

  Laboratoire de Cytologie, Institut des Neurosciences, CNRS, Université de Paris VI

• BRISORGUEIL Marie-Jeanne

  Laboratoire de Cytologie, Institut des Neurosciences, CNRS, Université de Paris VI

• ANGLADE Philippe

  Laboratoire de Physiopathologie et de Pathogenese des Maladies degeneratives du Systeme Nerveux

• ARANEDA Silvia

  Laboratoire de Cytologie, Institut des Neurosciences, CNRS, Université de Paris VI

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• Staining of acetylcholinesterase activity of the enteric nervous system by means of intracardiac perfusion of the histochemical medium : Restricted localization of the extracellular enzymatic activity
• A Combined Method of Immunocytochemistry of Choline-acetyltransferase Detection and of Autoradiography of (3H)choline after Retrograde Axonal Transport. Study of Cholinergic Pathways in the Central Nervous System at Light and Electron Microscopic Level.:S

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