A New Method for Isolation of Rh Polypeptide mRNAs Using a Liquid Culture System.

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概要

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• A selective two phase liquid culture system for erythroid progenitors was developed to isolate and analyze mRNAs encoding erythrocyte-specific blood group antigens. The feasibility of the technique was verified by isolation and identification of mRNA for Rh polypeptide using polymerase chain reaction accompanied with the subcloning and sequencing methods. Two kinds of Rh polypeptide-specific cDNA sequences were demonstrated. It was revealed that each individual has one or both of them. This approach based on the culture system is widely applicable to studies to detect gene expression of any proteins of erythroid blood group antigens at the RNA level.

• 日本学士院の論文

著者

• IKEMOTO Shigenori

  Department of Legal Medicine and Human Genetics, Jichi Medical School

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• A New Method for Isolation of Rh Polypeptide mRNAs Using a Liquid Culture System.

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