Effect of Dimethylsulfoxide on the Crystallization of Aspergillus niger Proteinase A.

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概要

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• Proteinase A from Aspergillus niger var. macrosporus is a non-pepsintype acid proteinase, whose catalytic site and enzymatic mechanism remain to be clarified. As the first step toward elucidating its three-dimensional structure by X-ray crystallography, proteinase A was crystallized in ammonium sulfate solutions by the hanging-drop vapor diffusion method. In the absence of an organic solvent, only the aggregates of long, thin needles were formed. On the other hand, thicker rodlike single crystals were grown at around pH 1.75 when dimethylsulfoxide (DMSO) was added to a concentration of around 5%. The single crystals of proteinase A thus obtained were suitable for X-ray diffraction measurements with a precession camera.

• 日本学士院の論文

著者

• TANOKURA Masaru

  Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo

関連論文

• Effect of Dimethylsulfoxide on the Crystallization of Aspergillus niger Proteinase A.

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