Growth of Mycobacterium leprae in vitro
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概要
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Mycobacterium leprae is cultured in vitro with a newly devised ML medium. Two different samples were tested, one M. leprae Thai- 53 strain which was passaged through nude mice in 1976, and another clinically isolated M. leprae K-1 strain. 108 cells from these samples were cultured with ML medium at 32°C in glass flasks, and the medium gradually become turbid (as measured by optical density (OD) at 510nm). The addition of ethylene oxide treated Cutina LE (Henkel, Germany) to the ML medium significantly increased the turbidity. The turbidity increase without Cutina LE was from 0.339 to 0.687 in 65 days, while the addition of Cutina LE resulted in OD change from 0.05 to 0.67 in 1 week. The observation of the surface of Cutina LE by phase-contrast microscope showed the adherent bacteria with apparent active multiplication. The identity of the turbidity was confirmed to be M. leprae by the use of Polymerase chain reaction.
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