EPON-FREEZE CRACKING FOR SCANNING ELECTRON MICROSCOPY

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概要

• 論文の詳細を見る

• Freeze cracking was carried out to visualize intracellular structures with scanning electron microscope (SEM). Tissues embedded in Epon 812 without any catalyst were frozen in liquid nitrogen and cracked at room temperature. After removal of the epoxy resin by displacing with propylene oxide, specimens were dried by critical point drying method. Then, the cracked face was ion-etched prior to coating with gold. The cracking technique was very easy, and the well-preserved structures of some organelles were demonstrated on the fractured faces thus obtained.

• 久留米大学医学部 The Kurume Medical Journal 編集部の論文

著者

• SHIMADA TATSUO

  Third Department of Internal Medicine, Kurume University School of Medicine

• OTA KATSUTOSHI

  Second Department of Anatomy, Kurume University School of Medicine

• YAMAMOTO MASATOSHI

  Third Department of Internal Medicine, Kurume University School of Medicine

• MURAKAMI MASAHIRO

  Third Department of Internal Medicine, Kurume University School of Medicine

関連論文

• EPON-FREEZE CRACKING FOR SCANNING ELECTRON MICROSCOPY

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