血液アミノ酸に關する研究 : 第1報血球分離術式

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Hitherto very limited studies have been undertaken for separation and purification of human leukocytes and blood platelets, except for cytochemical studies and other special purposes, due chiefly to the difficulty in the technique for obtaining these formed elements in pure and intact condition and in quantities ample enough for the studies.The authors method consists in using liquid paraffin for the non-wetting surface, subsurface transfer technique having been applied in a closed system.The centrifugal fractionation is carried out at the optimal temperature of 4°C and in shortest possible time. For leukocyte separation Cohns fraction I has been added, utilizing the albumin flotation method occasionally.By this method, not at all inferior to the methods employed by other workers, the leukocytes gave fair results and the blood platelets almost ideal results, as illustrated in Figs. 1-6.Using the materials as prepared by the authors method, examination of amino acids and other biochemical studies have been made.Although the purity of each fraction is satisfactory to a certain degree, such requirments as the recovery rate, freshness and intactness of the cellular elements must further be considered.The improvement in the method is now being made.
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