Enhanced Expression of bcl-2 Inhibits Apoptosis in Cultured Human Keratinocytes.

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概要

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• The molecular mechanisms regulating epidermal differentiation and apoptosis have not been elucidated. Bcl-2, one of the candidate genes for suppressing apoptosis, was originally cloned from the breakpoint of a t (14;18) translocation present in many human B cell lymphomas. In this study, the influence of bcl-2 on apoptosis was observed in transfected keratinocytes. After transfection of pEF-BOS vector withlwithout bcl-2, the expression of coded protein and the viability under starved conditions were examined. The bcl-2-transfected keratinocytes had cytoplasmic positive staining with anti-bcl-2 monoclonal antibodies, however the vector only transfected cells were devoid of the reaction products. The viability of transfected keratinocytes under starved conditions, with a lack of epidermal growth factor and bovine pituitary extract, was maintained in bcl-2 trans-fected cells; while the vector only transfected cells showed apoptotic cell death. The present result indicates that bcl-2 suppresses apoptotic cell death under starved conditions due to a lack of epidermal growth factor and bovine pituitary extract.

• 久留米大学医学部 The Kurume Medical Journal 編集部の論文

著者

• MAEYAMA YASUHIKO

  Department of Dermatology, Kurume University School of Medicine

関連論文

• The Effect of Orally-Administered Retinoid on Lectin Binding in Guinea Pig Keratinocytes.
• Cell Cycle Analyses of Extramammary Paget's Disease.
• Enhanced Expression of bcl-2 Inhibits Apoptosis in Cultured Human Keratinocytes.

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